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<h1 id="firstHeading" class="firstHeading mw-first-heading"><span class="mw-page-title-main">Lithiumborat-Puffer</span></h1>
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<div id="mw-content-text" class="mw-body-content mw-content-ltr" lang="de" dir="ltr"><div class="mw-content-ltr mw-parser-output" lang="de" dir="ltr"><p><b>Lithiumborat-Puffer</b> (<i>LB-Puffer</i>) ist ein <a href="Elektrophoresepuffer" title="Elektrophoresepuffer">Elektrophoresepuffer</a>, der in der <a href="Biochemie" title="Biochemie">Biochemie</a> und <a href="Molekularbiologie" title="Molekularbiologie">Molekularbiologie</a> im Zuge einer <a href="Agarose-Gelelektrophorese" title="Agarose-Gelelektrophorese">Agarose-Gelelektrophorese</a> zur Trennung von <a href="Nukleins%C3%A4ure" class="mw-redirect" title="Nukleinsäure">Nukleinsäuren</a> wie <a href="DNA" class="mw-redirect" title="DNA">DNA</a> oder <a href="RNA" class="mw-redirect" title="RNA">RNA</a> verwendet wird.<sup id="cite_ref-1" class="reference"><a href="#cite_note-1"><span class="cite-bracket">[</span>1<span class="cite-bracket">]</span></a></sup><sup id="cite_ref-2" class="reference"><a href="#cite_note-2"><span class="cite-bracket">[</span>2<span class="cite-bracket">]</span></a></sup><sup id="cite_ref-3" class="reference"><a href="#cite_note-3"><span class="cite-bracket">[</span>3<span class="cite-bracket">]</span></a></sup>
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<div class="mw-heading mw-heading2"><h2 id="Eigenschaften">Eigenschaften</h2></div>
<p>Der LB-Puffer ist aus <a href="Lithiumhydroxid" title="Lithiumhydroxid">Lithiumhydroxid</a> und <a href="Bors%C3%A4ure" title="Borsäure">Borsäure</a> zusammengesetzt und besitzt im Vergleich zu den alternativen Puffern (<a href="TAE-Puffer" title="TAE-Puffer">TAE-Puffer</a>, <a href="TBE-Puffer" title="TBE-Puffer">TBE-Puffer</a>, <a href="TPE-Puffer" title="TPE-Puffer">TPE-Puffer</a>, <a href="Natriumborat-Puffer" title="Natriumborat-Puffer">Natriumborat-Puffer</a>) eine niedrigere <a href="Ionenst%C3%A4rke" title="Ionenstärke">Ionenstärke</a> und daher eine niedrigere elektrische <a href="Leitf%C3%A4higkeit" title="Leitfähigkeit">Leitfähigkeit</a>. Dies erlaubt eine höhere angelegte <a href="Elektrische_Spannung" title="Elektrische Spannung">elektrische Spannung</a> und daher eine höhere Wanderungsgeschwindigkeit der Nukleinsäuren ohne Erhitzung des <a href="Agarosegel" class="mw-redirect" title="Agarosegel">Agarosegels</a>. Dadurch verkürzt sich die zur elektrophoretischen Trennung benötigte Zeit. Allerdings sind Lithiumsalze kostenintensiver. In den USA erhielt 2005 die Firma <i>Faster Better Media LLC</i> ein Patent auf den LB-Puffer.<sup id="cite_ref-4" class="reference"><a href="#cite_note-4"><span class="cite-bracket">[</span>4<span class="cite-bracket">]</span></a></sup>
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<div class="mw-heading mw-heading2"><h2 id="Weblinks">Weblinks</h2></div>
<ul><li><a rel="nofollow" class="external text" href="http://lclane.net/text/borate.html">Natriumborat als Elektrophoresepuffer</a></li></ul>
<div class="mw-heading mw-heading2"><h2 id="Einzelnachweise">Einzelnachweise</h2></div>
<ol class="references">
<li id="cite_note-1"><span class="mw-cite-backlink"><a href="#cite_ref-1">↑</a></span> <span class="reference-text">Jonathan R. Brody, Scott E. Kern: <i>Sodium boric acid: a tris-free, cooler conductive medium for DNA electrophoresis.</i> In: <i>BioTechniques.</i> Bd. 36, Nr. 2, 2004, <span class="-print"><a href="Internationale_Standardnummer_f%C3%BCr_fortlaufende_Sammelwerke" title="Internationale Standardnummer für fortlaufende Sammelwerke">ISSN</a> <span style="white-space:nowrap"><a rel="nofollow" class="external text" href="https://zdb-katalog.de/list.xhtml?t=iss%3D%220736-6205%22&key=cql">0736-6205</a></span></span>, S. 214–215, <a class="external mw-magiclink-pmid" rel="nofollow" href="https://www.ncbi.nlm.nih.gov/pubmed/14989083?dopt=Abstract">PMID 14989083</a> <style data-mw-deduplicate="TemplateStyles:r261891140">
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</style><a rel="nofollow" class="external text" href="https://web.archive.org/web/20110724175053/http://www.biotechniques.com/multimedia/archive/00036/BTN_A_04362BM02_O_36180a.pdf">(PDF; 155 kB)</a> (<span class="webarchiv-memento"><a href="Webarchivierung#Begrifflichkeiten" title="Webarchivierung">Memento</a></span> vom 24. Juli 2011 im <i><a href="Internet_Archive" title="Internet Archive">Internet Archive</a></i>).</span>
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<li id="cite_note-2"><span class="mw-cite-backlink"><a href="#cite_ref-2">↑</a></span> <span class="reference-text">Jonathan R. Brody, Eric S. Calhoun, Eike Gallmeier, Talisa D. Creavalle, Scott E. Kern (2004): <i>Ultra-fast high-resolution agarose electrophoresis of DNA and RNA using low-molarity conductive media.</i> In: <i>BioTechniques.</i> Bd. 37, Nr. 4, 2004, S. 598–602, <a class="external mw-magiclink-pmid" rel="nofollow" href="https://www.ncbi.nlm.nih.gov/pubmed/15517972?dopt=Abstract">PMID 15517972</a> <a rel="nofollow" class="external text" href="http://www.biotechniques.com/biotechniques/multimedia/archive/00037/BTN_A_04374ST04_O_37093a.pdf">(PDF; 198 kB)</a><span style="display:none"><a rel="nofollow" class="external text" href="http://deadurl.invalid/http://www.biotechniques.com/biotechniques/multimedia/archive/00037/BTN_A_04374ST04_O_37093a.pdf">@1</a></span><span style="display:none"><a rel="nofollow" class="external text" href="http://www.biotechniques.com/biotechniques/multimedia/archive/00037/BTN_A_04374ST04_O_37093a.pdf">@2</a></span><span style="display:none">Vorlage:Toter Link/www.biotechniques.com</span> <small>(Seite nicht mehr abrufbar, festgestellt im April 2019. <a rel="nofollow" class="external text" href="http://timetravel.mementoweb.org/list/2010/http://www.biotechniques.com/biotechniques/multimedia/archive/00037/BTN_A_04374ST04_O_37093a.pdf">Suche in Webarchiven</a>)</small></span>
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<li id="cite_note-3"><span class="mw-cite-backlink"><a href="#cite_ref-3">↑</a></span> <span class="reference-text">Jonathan R. Brody, Scott E. Kern: <i>History and principles of conductive media for standard DNA electrophoresis.</i> In: <i><a href="Analytical_Biochemistry" title="Analytical Biochemistry">Analytical Biochemistry</a>.</i> Bd. 333, Nr. 1, 2004, S. 1–13, <a class="external mw-magiclink-pmid" rel="nofollow" href="https://www.ncbi.nlm.nih.gov/pubmed/15351274?dopt=Abstract">PMID 15351274</a> <a rel="nofollow" class="external text" href="http://www.cc.ahs.chula.ac.th/Molmed/DNA%20electro%27s%20conductive%20media.pdf">(PDF; 1,14 MB)</a>.</span>
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<li id="cite_note-4"><span class="mw-cite-backlink"><a href="#cite_ref-4">↑</a></span> <span class="reference-text"><span class="cite">Patent <a rel="nofollow" class="external text" href="https://worldwide.espacenet.com/publicationDetails/biblio?locale=de_EP&CC=US&NR=7163610">US7163610</a>: <i>Conductive media for electrophoresis.</i> Veröffentlicht am <span style="white-space:nowrap;">26. Mai 2005</span>, Anmelder: Faster Better Media LLC.</span><span class="Z3988" title="ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Apatent&rft_id=US7163610&rft.applcc=US&rft.title=Conductive+media+for+electrophoresis&rft.assignee=Faster+Better+Media+LLC&rft.pubdate=2005-05-26"></span></span>
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